A method for the study of fungal growth inhibition by plant proteins.
نویسندگان
چکیده
A bioassay is described for the study of inhibitory activity of plant proteins on fungal growth. Fungal spores were germinated in liquid growth medium and pipetted into wells of a microtitre plate. Fungal growth was followed spectrophotometrically. The bioassay was tested using crude protein extracts from plant tissues known to have high activities of chitinase and beta-1,3-glucanase, and with purified enzymes. Crude protein preparations and combinations of the purified enzymes produced a temporary reduction of growth but no permanent growth inhibition.
منابع مشابه
In vitro anti-fungal activity of watercress (Nasturtium officinale) extract against Fusarium solani, the causal agent of potato dry rot
Background & Aim: The aim of this study was to assess the antifungal effect of the watercress extract on the growth of Fusarium solani, the causal agent of potato dry rot. Experimental: This research was done in a completely randomized design with three concentrations and four replicates on potato dextrose agar (PDA) culture medium. Results: Results of this study indicated that all ...
متن کاملIn vitro anti-fungal activity of watercress (Nasturtium officinale) extract against Fusarium solani, the causal agent of potato dry rot
Background & Aim: The aim of this study was to assess the antifungal effect of the watercress extract on the growth of Fusarium solani, the causal agent of potato dry rot. Experimental: This research was done in a completely randomized design with three concentrations and four replicates on potato dextrose agar (PDA) culture medium. Results: Results of this study indicated that all ...
متن کاملIn Vitro Anti-Mycotoxigenic and Anti-Aflatoxigenic Properties of Probiotic Bacteria; Lactobacillus plantarum and L. paracasei
The present study was designed to examine the effects of Lactobacillus plantaraum and L. paracasei on the growth and aflatoxin-producing ability of Aspergillus parasiticus. In these experiments, direct and indirect interactions of two different probiotic lactobacilli with A. parasiticus was examined.A co-culture system which supported both fungal growth and probiotic bacteria showed the inhibit...
متن کاملSynergistic Antifungal Effects of Lactobacillus Strains and Nano Selenium on Growth Inhibition of Candida albicans
Background and Aims: Candidiasis is a fungal infection caused by Candida albicans. Uncontrolled use of antibiotics has resulted in drug resistance. This study has been conducted to find a suitable alternative for these drugs. Materials and Methods: Primarily, standard and clinical isolates of C.albicans were collected. In order to indentify clinical isolates from lambs, the conventional mycolo...
متن کاملBiosynthesized Zinc Oxide nanoparticles control the growth of Aspergillus flavus and its aflatoxin production
The infection of Aspergillus flavus and its aflatoxin production pose a severe threat to humans, animals as well as plants life. Their inhibition using green techniques are considered as one of the important challenges. The present study outlines the antifungal activity of the ZnO nanoparticles (NPs) synthesized from lemongrass leaf extract and their effect on the mycelial growth of <e...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- FEMS microbiology letters
دوره 57 1-2 شماره
صفحات -
تاریخ انتشار 1990